Journal: Neuroscience research

Article Title: Roles of 5-HT 1A receptor in the expression of AMPA receptor and BDNF in developing mouse cortical neurons.

doi: 10.1016/j.neures.2016.09.008

Figure Lengend Snippet: Fig. 1. Expression of 5-HT1A receptor and AMPA receptor subunit GluR1 in cortical neurons cultured for 3 days (A and B) and 14 days (C and D). (A) Neurons at 3 DIV were stained

Article Snippet: To examine the localization of 5-HT1A receptor and GluR1 receptor, cortical neurons at 3 DIV and 14 DIV were incubated overnight at 4 ◦C with the rat anti-5-HT1A receptor antibody (1:1000 dilution) and rabbit anti-GluR1 antibody (#13185, 1:200 dilution, Cell Signaling Technology, U.S.A.), followed by the incubation with Alexa Fluor 488-conjugated goat anti-rat IgG antibody and Alexa Fluor 594-conjugated goat anti-rabbit IgG antibody for 1 h at room temperature.

Techniques: Expressing, Cell Culture, Staining

Journal: Neuroscience research

Article Title: Roles of 5-HT 1A receptor in the expression of AMPA receptor and BDNF in developing mouse cortical neurons.

doi: 10.1016/j.neures.2016.09.008

Figure Lengend Snippet: Fig. 2. Effects of 5-HT1A receptor agonist 8-OH-DPAT on the mRNA expression of BDNF and AMPA receptor subunits, GluR1 and GluR2, in cortical neurons in vitro. Neurons were

Article Snippet: To examine the localization of 5-HT1A receptor and GluR1 receptor, cortical neurons at 3 DIV and 14 DIV were incubated overnight at 4 ◦C with the rat anti-5-HT1A receptor antibody (1:1000 dilution) and rabbit anti-GluR1 antibody (#13185, 1:200 dilution, Cell Signaling Technology, U.S.A.), followed by the incubation with Alexa Fluor 488-conjugated goat anti-rat IgG antibody and Alexa Fluor 594-conjugated goat anti-rabbit IgG antibody for 1 h at room temperature.

Techniques: Expressing, In Vitro

Journal: Neuroscience research

Article Title: Roles of 5-HT 1A receptor in the expression of AMPA receptor and BDNF in developing mouse cortical neurons.

doi: 10.1016/j.neures.2016.09.008

Figure Lengend Snippet: Fig. 3. Effects of 5-HT1A receptor agonist 8-OH-DPAT on the mRNA expression of BDNF and AMPA receptor subunits, GluR1 and GluR2, in the frontal cortex (A), and Tph2 and 5-HTT

Article Snippet: To examine the localization of 5-HT1A receptor and GluR1 receptor, cortical neurons at 3 DIV and 14 DIV were incubated overnight at 4 ◦C with the rat anti-5-HT1A receptor antibody (1:1000 dilution) and rabbit anti-GluR1 antibody (#13185, 1:200 dilution, Cell Signaling Technology, U.S.A.), followed by the incubation with Alexa Fluor 488-conjugated goat anti-rat IgG antibody and Alexa Fluor 594-conjugated goat anti-rabbit IgG antibody for 1 h at room temperature.

Techniques: Expressing

Journal: Cell death & disease

Article Title: Functional validation of a human GLUD2 variant in a murine model of Parkinson's disease.

doi: 10.1038/s41419-020-03043-2

Figure Lengend Snippet: Fig. 3 GLUD2 mutation decreases glutamate transporter expression in MPTP-treated mice. a Expression levels of NR2A, GluA1, GluA2, EAAT1, and EAAT2 in the SN of AAV-GFP, MPTP + AAV-GFP, MPTP + AAV-GLUD2, and MPTP + AAV-GLUD2 T1492G groups of mice were determined by western blotting. n = 6. b, c Immunofluorescent staining of GFP, GFAP, and EAAT1 or EAAT2 in the SNpc of AAV-GFP, MPTP + AAV-GFP, MPTP + AAV- GLUD2, and MPTP + AAV-GLUD2 T1492G groups (scale bars: upper, 40 µm; lower, 8 µm). d Effect of GLUD2 or its mutant on the expression of GDH2, GDH1, GFAP, EAAT1 and EAAT2 in MPP+-treated U251 cells was determined by western blotting. The bottom band (~55 KD) in the GDH2 blot is non- specific. n = 3. Results are expressed as the mean ± SEM. **p < 0.01, *p < 0.05 vs. AAV-GFP group or untreated U251 cells. ##p < 0.01 vs. MPTP + AAV- GFP or MPP+ group. &&p < 0.01, &p < 0.05 vs. MPP+ + GLUD2 group. Statistical significance was determined by one-way ANOVAs and Tukey tests for post-hoc comparisons.

Article Snippet: Anti-GFAP (#80788), NR2A (#4205), GluA1 (#13185), Bax (#14796), and GluA2 (#13607) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Mutagenesis, Expressing, Western Blot, Staining